HPLC & COA
HPLC + MS verification, on every lot we ship.
No batch reuses a previous Certificate of Analysis. Every analytical claim — purity, identity, residual solvents, water content — applies to the exact vial you receive, traceable by lot number.
What HPLC measures
High-Performance Liquid Chromatography separates the contents of a sample by retention time on a stationary phase. For a synthesized peptide, the chromatogram shows the target peptide as the main peak plus any synthesis byproducts, truncated sequences, racemized residues, and protecting-group leftovers as smaller peaks.
≥99% purity by HPLC means the target peptide peak accounts for ≥99% of the total chromatogram area. The remaining ≤1% is composed of related impurities. For research-grade reference compounds intended for in-vitro work, this threshold is the practical line between “characterizable” and “qualitative-only.”
Analytical pipeline — per lot
How to read a Kinotype COA
- Lot number — top-right of the document. Matches the lot printed on the vial label. Cross-reference before any experimental run.
- Compound + CAS number — identity declaration.
- Purity (HPLC) — the headline number with the integrated area percentage.
- HPLC chromatogram — the actual trace. The target peak should dominate; impurity peaks should be minor.
- MS spectrum — observed vs. theoretical mass.
- Storage + expiry — recommended storage condition and lot expiry date.
- Analyst signature + date — the lab that ran the analysis.
Where to find your COA
Every order includes a printed COA matching the lot numbers of vials in the box.
Sign in and the COA PDF for each order is linked from the order detail page.
Each label has a QR that resolves to the COA URL for that lot number.
Public batch certificates
Sample COAs for the most recent batch of each catalog compound. Your specific order will include the COA matching your actual lot.
Each card will link to the PDF for that compound’s representative lot once uploaded via Media Library.
What HPLC doesn’t tell you
HPLC purity is necessary but not sufficient. A vial can be 99% pure by HPLC and still have practical issues:
- Bacterial contamination — HPLC doesn’t measure microbial load. Sterility is enforced via cleanroom synthesis + final filtration.
- Endotoxins — measured by separate LAL assay when required.
- Folding / aggregation — primary structure can be correct while tertiary structure is off. Empirical, not chromatographic.
For assay-level validation beyond HPLC + MS, contact us before ordering and we can arrange additional analytical work per batch.